The core facility provides next-gen sequencing, microarray, and quantitative PCR services, training and support. Consultation is available to all researchers at the Institute as well as external institutions and collaborators.
Systems biology approaches entail simultaneous measurements under numerous different biological conditions and time points. The core houses various instruments to enable this high-throughput research. In addition to a NextSeq for RNAseq, small RNAseq and ChIPseq projects, the facility supports Agilent and Exiqon microarrays for RNA and miRNA measurements. Single cell analysis can be performed by the Chromium 10x in conjunction with the NextSeq. Also available are two real-time PCR instruments (an ABI 7900HT and a QuantStudio 6 Flex), the Fluidigm system (consisting of the Biomark for high troughput rtPCR with both the HT and LT controller, for 48.48 assays and 96.96 assays ), and the Nanostring nCounter for mRNA, DNA and miRNA expression measurements.
Remember to acknowledge the valuable services provided by Gene Expression Core Facility in your research papers and publications: “This research was supported by the Gene Expression Core Facility of the Institute for Systems Biology.”
For information on pricing and scheduling, email firstname.lastname@example.org,
or call (206)732-1228 / -1347.